| 1. Jornada | 25.07.2021 | Spartak Trnava | - | FK Pohronie | 2:0 | ||||
| 2. Jornada | 01.08.2021 | FK Senica | - | Spartak Trnava | 2:1 | ||||
| 3. Jornada | 09.08.2021 | Spartak Trnava | - | Tatran Liptovský Mikuláš | 4:0 | ||||
| 4. Jornada | 15.08.2021 | Spartak Trnava | - | Zlaté Moravce | 2:0 | ||||
| 5. Jornada | 21.08.2021 | MFK Ružomberok | - | Spartak Trnava | 0:0 | ||||
| 6. Jornada | 29.08.2021 | Spartak Trnava | - | MŠK Žilina | 1:1 |  |
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| 7. Jornada | 11.09.2021 | MFK Zemplín Michalovce | - | Spartak Trnava | 1:2 | |
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| 8. Jornada | 17.09.2021 | Spartak Trnava | - | ŠKF Sereď | 2:0 | |
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| 9. Jornada | 26.09.2021 | FC DAC 1904 | - | Spartak Trnava | 0:3 | ||||
| 10. Jornada | 03.10.2021 | AS Trenčín | - | Spartak Trnava | 0:1 | |
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| 11. Jornada | 17.10.2021 | Spartak Trnava | - | Slovan Bratislava | 0:3 | ||||
| 17. Jornada | 05.12.2021 | MŠK Žilina | - | Spartak Trnava | 0:1 | ||||
| 18. Jornada | 10.12.2021 | Spartak Trnava | - | MFK Zemplín Michalovce | 2:0 | ||||
| 19. Jornada | 18.12.2021 | ŠKF Sereď | - | Spartak Trnava | 0:1 |
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