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| 02.05.2009 | 19:00 | Tescoma Zlín | - | Bohemians Praha | 2:0 (1:0) | |
| 03.05.2009 | 10:15 | Viktoria Žižkov | - | Dynamo České Budějovice | 2:1 (1:1) | |
| 17:00 | Sigma Olomouc | - | FK Teplice | 2:1 (1:0) | ||
| 17:00 | SK Kladno | - | 1. FK Příbram | 1:0 (1:0) | ||
| 17:00 | FK Baumit Jablonec | - | Viktoria Plzeň | 3:0 (1:0) | ||
| 17:00 | 1. FC Brno | - | Slavia Praga | 1:0 (1:0) | ||
| 20:00 | FK Mladá Boleslav | - | Slovan Liberec | 1:4 (1:1) | ||
| 04.05.2009 | 17:15 | Sparta Praga | - | Baník Ostrava | 1:0 (0:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praga | 26 | 15 | 7 | 4 | 49:22 | 27 | 52 |
| 2 |
|
Sparta Praga | 26 | 13 | 8 | 5 | 40:21 | 19 | 47 |
| 3 |
|
Slovan Liberec | 26 | 12 | 8 | 6 | 35:25 | 10 | 44 |
| 4 |
|
Sigma Olomouc | 26 | 12 | 7 | 7 | 33:32 | 1 | 43 |
| 5 |
|
FK Mladá Boleslav | 26 | 11 | 9 | 6 | 35:32 | 3 | 42 |
| 6 |
|
FK Baumit Jablonec | 26 | 12 | 4 | 10 | 28:29 | -1 | 40 |
| 7 |
|
FK Teplice | 26 | 11 | 6 | 9 | 30:19 | 11 | 39 |
| 8 |
|
Baník Ostrava | 26 | 10 | 5 | 11 | 34:30 | 4 | 35 |
| 9 |
|
1. FC Brno | 26 | 9 | 7 | 10 | 27:28 | -1 | 34 |
| 10 |
|
Viktoria Plzeň | 26 | 8 | 9 | 9 | 38:35 | 3 | 33 |
| 11 |
|
Dynamo České Budějovice | 26 | 5 | 15 | 6 | 24:31 | -7 | 30 |
| 12 |
|
SK Kladno | 26 | 8 | 6 | 12 | 20:37 | -17 | 30 |
| 13 |
|
Bohemians Praha | 26 | 8 | 4 | 14 | 27:38 | -11 | 28 |
| 14 |
|
1. FK Příbram | 26 | 6 | 7 | 13 | 23:34 | -11 | 25 |
| 15 |
|
Viktoria Žižkov | 26 | 5 | 7 | 14 | 25:35 | -10 | 22 |
| 16 |
|
Tescoma Zlín | 26 | 5 | 7 | 14 | 20:40 | -20 | 22 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
