| 1. Jornada | 24.07.2021 | FK Pardubice | - | MFK Karviná | 2:2 | ||||
| 2. Jornada | 31.07.2021 | MFK Karviná | - | FC Hradec Králové | 1:1 | ||||
| 4. Jornada | 14.08.2021 | MFK Karviná | - | FC Fastav Zlín | 2:3 | ||||
| 5. Jornada | 22.08.2021 | Viktoria Plzeň | - | MFK Karviná | 2:0 | ||||
| 8. Jornada | 19.09.2021 | MFK Karviná | - | FK Teplice | 1:1 | ||||
| 10. Jornada | 03.10.2021 | MFK Karviná | - | FK Mladá Boleslav | 0:1 | ||||
| 11. Jornada | 17.10.2021 | Sigma Olomouc | - | MFK Karviná | 2:0 | ||||
| 14. Jornada | 07.11.2021 | MFK Karviná | - | 1. FC Slovácko | 2:2 | ||||
| 15. Jornada | 21.11.2021 | MFK Karviná | - | Slovan Liberec | 1:2 | ||||
| 12. Jornada | 24.11.2021 | MFK Karviná | - | Baník Ostrava | 1:2 | ||||
| 16. Jornada | 28.11.2021 | FC Hradec Králové | - | MFK Karviná | 1:0 | ||||
| 13. Jornada | 01.12.2021 | Bohemians Praha 1905 | - | MFK Karviná | 3:0 | ||||
| 17. Jornada | 04.12.2021 | MFK Karviná | - | Sparta Praga | 1:2 | ||||
| 18. Jornada | 11.12.2021 | FC Fastav Zlín | - | MFK Karviná | 2:1 | ||||
| 19. Jornada | 18.12.2021 | MFK Karviná | - | Viktoria Plzeň | 0:1 |
República Checa