| 2. Jornada | 22.08.2016 | Vitesse (J) | - | Jong FC Twente | 1:3 | ||||
| 3. Jornada | 28.08.2016 | Jong FC Twente | - | TEC VV | 2:3 |  |
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| 5. Jornada | 10.09.2016 | Jong FC Twente | - | BVV Barendrecht | 0:0 | ||||
| 7. Jornada | 25.09.2016 | Jong FC Twente | - | VV Sint Bavo | 0:2 | ||||
| 8. Jornada | 03.10.2016 | Sparta Rotterdam (J) | - | Jong FC Twente | 4:2 | ||||
| 9. Jornada | 07.10.2016 | Jong FC Twente | - | AZ Alkmaar (J) | 2:3 |  |
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| 11. Jornada | 29.10.2016 | Jong FC Twente | - | HHC Hardenberg | 0:1 | ||||
| 12. Jornada | 05.11.2016 | Kozakken Boys | - | Jong FC Twente | 2:0 | ||||
| 17. Jornada | 10.12.2016 | Jong FC Twente | - | Koninklijke HFC | 1:1 |
Países Bajos