| 3. Jornada | 19.08.2023 | FK Baník Most-Souš | - | FK Kolín | 0:0 |  |
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| 4. Jornada | 26.08.2023 | FK Kolín | - | FK Teplice B | 1:1 | ||||
| 5. Jornada | 02.09.2023 | FK Jablonec B | - | FK Kolín | 3:1 | ||||
| 6. Jornada | 09.09.2023 | FK Kolín | - | Slovan Velvary | 0:4 | ||||
| 1. Jornada | 13.09.2023 | Sokol Živanice | - | FK Kolín | 3:0 | ||||
| 7. Jornada | 17.09.2023 | FK Mlada Boleslav B | - | FK Kolín | 1:2 | |
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| 8. Jornada | 22.09.2023 | FK Kolín | - | FK Přepeře | 6:1 | ||||
| 9. Jornada | 01.10.2023 | FC Hradec Králové B | - | FK Kolín | 1:1 | ||||
| 10. Jornada | 07.10.2023 | FK Kolín | - | SK Zápy | 2:2 | ||||
| 11. Jornada | 15.10.2023 | Slovan Liberec B | - | FK Kolín | 0:0 | ||||
| 12. Jornada | 21.10.2023 | FK Kolín | - | FK Ústí nad Labem | 3:2 | ||||
| 13. Jornada | 29.10.2023 | Chlumec nad Cidlinou | - | FK Kolín | 3:3 | ||||
| 14. Jornada | 04.11.2023 | FK Kolín | - | FK Pardubice B | 1:1 | ||||
| 15. Jornada | 11.11.2023 | Sokol Brozany | - | FK Kolín | 4:2 | |
República Checa