| 2. Jornada | 22.08.2016 | Vitesse (J) | - | Jong FC Twente | 1:3 |  |
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| 3. Jornada | 28.08.2016 | Jong FC Twente | - | TEC VV | 2:3 | ||||
| 5. Jornada | 10.09.2016 | Jong FC Twente | - | BVV Barendrecht | 0:0 | ||||
| 8. Jornada | 03.10.2016 | Sparta Rotterdam (J) | - | Jong FC Twente | 4:2 |  |
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| 9. Jornada | 07.10.2016 | Jong FC Twente | - | AZ Alkmaar (J) | 2:3 | ||||
| 10. Jornada | 15.10.2016 | VV Katwijk | - | Jong FC Twente | 0:1 | ||||
| 16. Jornada | 03.12.2016 | FC Lienden | - | Jong FC Twente | 1:1 | |
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| 21. Jornada | 28.01.2017 | Jong FC Twente | - | GVVV | 3:1 | |
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| 22. Jornada | 04.02.2017 | BVV Barendrecht | - | Jong FC Twente | 1:1 | |
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| 23. Jornada | 11.02.2017 | Jong FC Twente | - | VV Katwijk | 0:1 | ||||
| 25. Jornada | 05.03.2017 | VV UNA | - | Jong FC Twente | 3:1 | |
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| 26. Jornada | 11.03.2017 | Jong FC Twente | - | SV Spakenburg | 1:1 | ||||
| 28. Jornada | 27.03.2017 | Jong FC Twente | - | Sparta Rotterdam (J) | 0:3 |
Alemania