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| 30.07.2021 | 18:00 | FK Ústí nad Labem | - | Vysočina Jihlava | 1:2 (0:1) | |
| 31.07.2021 | 10:15 | FK Fotbal Třinec | - | FK Dukla Praha | 1:3 (1:1) | |
| 10:15 | MFK Vyškov | - | SK Líšeň | 1:1 (0:1) | ||
| 17:00 | 1. FK Příbram | - | FK MAS Táborsko | 0:2 (0:2) | ||
| 01.08.2021 | 10:30 | AC Sparta Praha B | - | 1. SK Prostějov | 4:0 (2:0) | |
| 17:00 | FK Varnsdorf | - | Viktoria Žižkov | 2:1 (1:1) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | MFK Chrudim | 4:1 (1:1) | ||
| 20:00 | Zbrojovka Brno | - | SFC Opava | 2:1 (0:1) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
AC Sparta Praha B | 2 | 2 | 0 | 0 | 5:0 | 5 | 6 |
| 2 |
|
FK Dukla Praha | 2 | 2 | 0 | 0 | 5:2 | 3 | 6 |
| 3 |
|
Vysočina Jihlava | 2 | 2 | 0 | 0 | 4:2 | 2 | 6 |
| 4 |
|
Zbrojovka Brno | 2 | 2 | 0 | 0 | 3:1 | 2 | 6 |
| 5 |
|
FC Sellier & Bellot Vlašim | 2 | 1 | 1 | 0 | 7:4 | 3 | 4 |
| 6 |
|
SK Líšeň | 2 | 1 | 1 | 0 | 4:3 | 1 | 4 |
| 7 |
|
FK MAS Táborsko | 2 | 1 | 0 | 1 | 2:1 | 1 | 3 |
| 8 |
|
FK Ústí nad Labem | 2 | 1 | 0 | 1 | 4:4 | 0 | 3 |
| 9 |
|
1. FK Příbram | 2 | 1 | 0 | 1 | 3:3 | 0 | 3 |
|
FK Varnsdorf | 2 | 1 | 0 | 1 | 3:3 | 0 | 3 | |
| 11 |
|
SFC Opava | 2 | 0 | 1 | 1 | 4:5 | -1 | 1 |
| 12 |
|
MFK Vyškov | 2 | 0 | 1 | 1 | 2:3 | -1 | 1 |
| 13 |
|
Viktoria Žižkov | 2 | 0 | 0 | 2 | 3:5 | -2 | 0 |
| 14 |
|
FK Fotbal Třinec | 2 | 0 | 0 | 2 | 3:6 | -3 | 0 |
| 15 |
|
MFK Chrudim | 2 | 0 | 0 | 2 | 1:5 | -4 | 0 |
| 16 |
|
1. SK Prostějov | 2 | 0 | 0 | 2 | 1:7 | -6 | 0 |
| Ascenso | |
| Playoff | |
| Descenso |
