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| 03.04.2001 | 16:30 | FC Marila Příbram | - | Sigma Olomouc | 0:0 (0:0) | |
| 16:30 | Slavia Praga | - | CU Bohemians Praha | 1:0 (0:0) | ||
| 04.04.2001 | 16:30 | Dynamo České Budějovice | - | Slovan Liberec | 0:0 (0:0) | |
| 16:30 | Chmel Blsany | - | 1. FC Synot Stare Mesto | 2:2 (0:2) | ||
| 16:30 | FK Jablonec 97 | - | Baník Ostrava | 2:1 (1:0) | ||
| 16:30 | FC Stavo Artikel Brno | - | FK Teplice | 2:0 (1:0) | ||
| 05.04.2001 | 16:30 | FK Drnovice | - | Viktoria Plzeň | 3:1 (1:0) | |
| 16:30 | Viktoria Žižkov | - | Sparta Praga | 2:1 (1:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praga | 22 | 16 | 3 | 3 | 55:22 | 33 | 51 |
| 2 |
|
Sigma Olomouc | 22 | 10 | 8 | 4 | 38:22 | 16 | 38 |
| 3 |
|
FC Marila Příbram | 22 | 10 | 6 | 6 | 32:22 | 10 | 36 |
| 4 |
|
Viktoria Žižkov | 22 | 9 | 7 | 6 | 35:33 | 2 | 34 |
| 5 |
|
FK Teplice | 22 | 10 | 3 | 9 | 35:27 | 8 | 33 |
| 6 |
|
Slavia Praga | 22 | 8 | 9 | 5 | 31:26 | 5 | 33 |
| 7 |
|
Slovan Liberec | 22 | 9 | 5 | 8 | 31:28 | 3 | 32 |
| 8 |
|
1. FC Synot Stare Mesto | 22 | 7 | 9 | 6 | 30:26 | 4 | 30 |
| 9 |
|
FK Drnovice | 22 | 8 | 6 | 8 | 25:25 | 0 | 30 |
| 10 |
|
FK Jablonec 97 | 22 | 7 | 7 | 8 | 21:29 | -8 | 28 |
| 11 |
|
CU Bohemians Praha | 22 | 6 | 9 | 7 | 27:28 | -1 | 27 |
| 12 |
|
FC Stavo Artikel Brno | 22 | 6 | 5 | 11 | 19:26 | -7 | 23 |
| 13 |
|
Baník Ostrava | 22 | 5 | 8 | 9 | 22:34 | -12 | 23 |
| 14 |
|
Chmel Blsany | 22 | 5 | 8 | 9 | 20:28 | -8 | 23 |
| 15 |
|
Dynamo České Budějovice | 22 | 4 | 7 | 11 | 27:46 | -19 | 19 |
| 16 |
|
Viktoria Plzeň | 22 | 3 | 6 | 13 | 19:45 | -26 | 15 |
