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| 14.03.2009 | 17:00 | Baník Ostrava | - | FK Teplice | 1:0 (0:0) | |
| 17:00 | Tescoma Zlín | - | Slovan Liberec | 3:3 (0:2) | ||
| 17:00 | Viktoria Plzeň | - | 1. FK Příbram | 0:0 (0:0) | ||
| 15.03.2009 | 17:00 | Sigma Olomouc | - | SK Kladno | 1:3 (0:1) | |
| 17:00 | Bohemians Praha | - | Viktoria Žižkov | 0:0 (0:0) | ||
| 17:00 | FK Mladá Boleslav | - | Dynamo České Budějovice | 0:0 (0:0) | ||
| 20:00 | FK Baumit Jablonec | - | Slavia Praga | 1:2 (0:1) | ||
| 16.03.2009 | 17:15 | Sparta Praga | - | 1. FC Brno | 4:0 (4:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praga | 20 | 14 | 5 | 1 | 43:14 | 29 | 47 |
| 2 |
|
Sparta Praga | 20 | 11 | 4 | 5 | 34:18 | 16 | 37 |
| 3 |
|
Baník Ostrava | 20 | 10 | 4 | 6 | 31:19 | 12 | 34 |
| 4 |
|
Slovan Liberec | 20 | 9 | 6 | 5 | 27:21 | 6 | 33 |
| 5 |
|
FK Mladá Boleslav | 20 | 9 | 6 | 5 | 28:24 | 4 | 33 |
| 6 |
|
FK Teplice | 20 | 9 | 4 | 7 | 24:15 | 9 | 31 |
| 7 |
|
FK Baumit Jablonec | 20 | 9 | 4 | 7 | 22:24 | -2 | 31 |
| 8 |
|
1. FC Brno | 20 | 7 | 6 | 7 | 23:22 | 1 | 27 |
| 9 |
|
Sigma Olomouc | 20 | 7 | 6 | 7 | 24:28 | -4 | 27 |
| 10 |
|
Dynamo České Budějovice | 20 | 4 | 12 | 4 | 17:24 | -7 | 24 |
| 11 |
|
Viktoria Plzeň | 20 | 5 | 8 | 7 | 25:27 | -2 | 23 |
| 12 |
|
Bohemians Praha | 20 | 6 | 4 | 10 | 23:30 | -7 | 22 |
| 13 |
|
1. FK Příbram | 20 | 5 | 5 | 10 | 19:27 | -8 | 20 |
| 14 |
|
SK Kladno | 20 | 5 | 5 | 10 | 14:30 | -16 | 20 |
| 15 |
|
Viktoria Žižkov | 20 | 3 | 5 | 12 | 18:29 | -11 | 14 |
| 16 |
|
Tescoma Zlín | 20 | 2 | 6 | 12 | 12:32 | -20 | 12 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
