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| 04.04.2009 | 17:00 | Tescoma Zlín | - | FK Teplice | 1:1 (1:0) | |
| 17:00 | Baník Ostrava | - | Viktoria Plzeň | 1:4 (0:1) | ||
| 05.04.2009 | 15:00 | FK Baumit Jablonec | - | Dynamo České Budějovice | 2:0 (1:0) | |
| 17:00 | Bohemians Praha | - | 1. FK Příbram | 1:0 (0:0) | ||
| 17:00 | SK Kladno | - | 1. FC Brno | 1:0 (0:0) | ||
| 17:00 | FK Mladá Boleslav | - | Viktoria Žižkov | 0:0 (0:0) | ||
| 20:00 | Sigma Olomouc | - | Slavia Praga | 1:0 (0:0) | ||
| 06.04.2009 | 17:15 | Sparta Praga | - | Slovan Liberec | 1:1 (0:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praga | 22 | 15 | 5 | 2 | 44:15 | 29 | 50 |
| 2 |
|
Sparta Praga | 22 | 11 | 6 | 5 | 35:19 | 16 | 39 |
| 3 |
|
Slovan Liberec | 22 | 10 | 7 | 5 | 30:23 | 7 | 37 |
| 4 |
|
FK Mladá Boleslav | 22 | 10 | 7 | 5 | 29:24 | 5 | 37 |
| 5 |
|
FK Baumit Jablonec | 22 | 11 | 4 | 7 | 25:24 | 1 | 37 |
| 6 |
|
Baník Ostrava | 22 | 10 | 4 | 8 | 33:25 | 8 | 34 |
| 7 |
|
FK Teplice | 22 | 9 | 6 | 7 | 25:16 | 9 | 33 |
| 8 |
|
Sigma Olomouc | 22 | 9 | 6 | 7 | 27:29 | -2 | 33 |
| 9 |
|
Viktoria Plzeň | 22 | 6 | 9 | 7 | 30:29 | 1 | 27 |
| 10 |
|
1. FC Brno | 22 | 7 | 6 | 9 | 24:25 | -1 | 27 |
| 11 |
|
Bohemians Praha | 22 | 7 | 4 | 11 | 25:32 | -7 | 25 |
| 12 |
|
Dynamo České Budějovice | 22 | 4 | 13 | 5 | 18:27 | -9 | 25 |
| 13 |
|
1. FK Příbram | 22 | 6 | 5 | 11 | 21:29 | -8 | 23 |
| 14 |
|
SK Kladno | 22 | 6 | 5 | 11 | 15:31 | -16 | 23 |
| 15 |
|
Viktoria Žižkov | 22 | 3 | 6 | 13 | 18:30 | -12 | 15 |
| 16 |
|
Tescoma Zlín | 22 | 2 | 7 | 13 | 13:34 | -21 | 13 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
