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| 12.09.2009 | 16:00 | Baník Ostrava | - | Bohemians Praha | 1:0 (1:0) | |
| 16:00 | 1. FK Příbram | - | Viktoria Plzeň | 1:0 (1:0) | ||
| 18:15 | Sparta Praga | - | FK Baumit Jablonec | 2:0 (0:0) | ||
| 20:00 | Slovan Liberec | - | Slavia Praga | 1:1 (1:1) | ||
| 13.09.2009 | 17:00 | Bohemians 1905 | - | Dynamo České Budějovice | 2:0 (0:0) | |
| 17:00 | SK Kladno | - | 1. FC Brno | 1:0 (0:0) | ||
| 19:00 | 1. FC Slovácko | - | FK Teplice | 0:2 (0:1) | ||
| 14.09.2009 | 17:30 | FK Mladá Boleslav | - | Sigma Olomouc | 2:2 (1:1) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Teplice | 7 | 4 | 3 | 0 | 14:6 | 8 | 15 |
| 2 |
|
Baník Ostrava | 7 | 4 | 2 | 1 | 8:5 | 3 | 14 |
| 3 |
|
FK Baumit Jablonec | 7 | 4 | 2 | 1 | 9:7 | 2 | 14 |
| 4 |
|
Sparta Praga | 7 | 3 | 4 | 0 | 7:2 | 5 | 13 |
| 5 |
|
Slavia Praga | 7 | 3 | 3 | 1 | 10:6 | 4 | 12 |
| 6 |
|
FK Mladá Boleslav | 7 | 3 | 2 | 2 | 12:7 | 5 | 11 |
| 7 |
|
1. FC Brno | 7 | 3 | 2 | 2 | 7:5 | 2 | 11 |
| 8 |
|
1. FK Příbram | 7 | 3 | 2 | 2 | 7:6 | 1 | 11 |
| 9 |
|
Viktoria Plzeň | 7 | 3 | 1 | 3 | 9:9 | 0 | 10 |
| 10 |
|
Bohemians 1905 | 7 | 2 | 3 | 2 | 4:3 | 1 | 9 |
| 11 |
|
Slovan Liberec | 7 | 2 | 2 | 3 | 10:11 | -1 | 8 |
| 12 |
|
Sigma Olomouc | 7 | 1 | 4 | 2 | 7:8 | -1 | 7 |
| 13 |
|
1. FC Slovácko | 7 | 2 | 0 | 5 | 8:12 | -4 | 6 |
| 14 |
|
SK Kladno | 7 | 1 | 1 | 5 | 4:11 | -7 | 4 |
| 15 |
|
Dynamo České Budějovice | 7 | 1 | 1 | 5 | 4:12 | -8 | 4 |
| 16 |
|
Bohemians Praha | 7 | 1 | 0 | 6 | 4:14 | -10 | -17 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
