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| 13.02.2009 | 20:00 | FC Den Bosch | - | Telstar | 3:0 (2:0) | |
| 20:00 | SBV Excelsior | - | MVV | 1:1 (0:1) | ||
| 20:00 | Fortuna Sittard | - | Go Ahead Eagles | 0:1 (0:0) | ||
| 20:00 | VVV-Venlo | - | SC Cambuur | 1:2 (1:0) | ||
| 20:00 | Helmond Sport | - | FC Emmen | 3:1 (1:0) | ||
| 20:00 | HFC Haarlem | - | FC Eindhoven | 2:1 (1:0) | ||
| 20:00 | BV Veendam | - | RKC Waalwijk | 0:2 (0:1) | ||
| 20:00 | FC Omniworld | - | TOP Oss | 0:2 (0:1) | ||
| 20:00 | FC Zwolle | - | RBC Roosendaal | 0:2 (0:2) | ||
| 20:00 | FC Dordrecht | - | AGOVV | 2:1 (2:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
VVV-Venlo | 26 | 18 | 3 | 5 | 63:27 | 36 | 57 |
| 2 |
|
RKC Waalwijk | 26 | 11 | 14 | 1 | 45:24 | 21 | 47 |
| 3 |
|
SC Cambuur | 26 | 13 | 5 | 8 | 48:39 | 9 | 44 |
| 4 |
|
SBV Excelsior | 26 | 11 | 10 | 5 | 40:24 | 16 | 43 |
| 5 |
|
FC Zwolle | 26 | 11 | 7 | 8 | 42:39 | 3 | 40 |
| 6 |
|
HFC Haarlem | 26 | 12 | 4 | 10 | 33:36 | -3 | 40 |
| 7 |
|
MVV | 26 | 10 | 9 | 7 | 39:29 | 10 | 39 |
| 8 |
|
Helmond Sport | 26 | 10 | 9 | 7 | 40:35 | 5 | 39 |
| 9 |
|
Go Ahead Eagles | 26 | 10 | 9 | 7 | 25:28 | -3 | 39 |
| 10 |
|
FC Den Bosch | 26 | 10 | 8 | 8 | 45:33 | 12 | 38 |
| 11 |
|
TOP Oss | 26 | 9 | 5 | 12 | 31:41 | -10 | 32 |
| 12 |
|
RBC Roosendaal | 26 | 8 | 7 | 11 | 31:33 | -2 | 31 |
| 13 |
|
AGOVV | 26 | 9 | 4 | 13 | 39:51 | -12 | 31 |
| 14 |
|
Telstar | 26 | 7 | 9 | 10 | 34:41 | -7 | 30 |
| 15 |
|
Fortuna Sittard | 26 | 7 | 7 | 12 | 28:41 | -13 | 28 |
| 16 |
|
FC Dordrecht | 26 | 6 | 8 | 12 | 29:32 | -3 | 26 |
| 17 |
|
FC Eindhoven | 26 | 6 | 11 | 9 | 43:53 | -10 | 26 |
| 18 |
|
BV Veendam | 26 | 7 | 8 | 11 | 31:42 | -11 | 26 |
| 19 |
|
FC Emmen | 26 | 6 | 6 | 14 | 32:53 | -21 | 24 |
| 20 |
|
FC Omniworld | 26 | 5 | 5 | 16 | 27:44 | -17 | 20 |
| Ascenso | |
| Playoff | |
| Playoff | |
| Playoff | |
| Playoff |
