| 25. Jornada | 14.08.2021 | FC Ryūkyū | - | Mito HollyHock | 0:1 | ||||
| 26. Jornada | 22.08.2021 | Mito HollyHock | - | Kyoto Sanga FC | 0:2 | ||||
| 27. Jornada | 28.08.2021 | Mito HollyHock | - | Albirex Niigata | 4:0 | ||||
| 28. Jornada | 04.09.2021 | Montedio Yamagata | - | Mito HollyHock | 0:1 | ||||
| 29. Jornada | 11.09.2021 | Mito HollyHock | - | Blaublitz Akita | 0:3 | ||||
| 30. Jornada | 18.09.2021 | Tochigi SC | - | Mito HollyHock | 0:0 | ||||
| 31. Jornada | 26.09.2021 | Mito HollyHock | - | Zweigen Kanazawa | 2:1 | ||||
| 33. Jornada | 10.10.2021 | Mito HollyHock | - | Thespakusatsu Gunma | 2:1 |  |
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| 36. Jornada | 30.10.2021 | V-Varen Nagasaki | - | Mito HollyHock | 2:1 | ||||
| 37. Jornada | 03.11.2021 | Mito HollyHock | - | Giravanz Kitakyushu | 0:0 | ||||
| 38. Jornada | 07.11.2021 | JEF United Chiba | - | Mito HollyHock | 2:2 | ||||
| 39. Jornada | 14.11.2021 | Mito HollyHock | - | Júbilo Iwata | 1:3 | ||||
| 40. Jornada | 21.11.2021 | RB Omiya Ardija | - | Mito HollyHock | 1:3 | ||||
| 41. Jornada | 28.11.2021 | Mito HollyHock | - | Ehime FC | 2:1 | ||||
| 42. Jornada | 05.12.2021 | Ventforet Kofu | - | Mito HollyHock | 3:3 |
Japón