| 1. Jornada | 12.09.2020 | FC Twente | - | Fortuna Sittard | 2:0 | ||||
| 2. Jornada | 20.09.2020 | Feyenoord | - | FC Twente | 1:1 |  |
|||
| 3. Jornada | 25.09.2020 | FC Twente | - | FC Groningen | 3:1 | |
|||
| 4. Jornada | 03.10.2020 | FC Twente | - | FC Emmen | 1:1 | |
 |
||
| 5. Jornada | 17.10.2020 | Willem II | - | FC Twente | 0:3 | ||||
| 6. Jornada | 24.10.2020 | FC Utrecht | - | FC Twente | 2:1 | ||||
| 7. Jornada | 31.10.2020 | FC Twente | - | PEC Zwolle | 5:1 | |
|||
| 8. Jornada | 07.11.2020 | ADO Den Haag | - | FC Twente | 2:4 | ||||
| 9. Jornada | 22.11.2020 | FC Twente | - | PSV | 1:1 | ||||
| 10. Jornada | 27.11.2020 | FC Twente | - | RKC Waalwijk | 0:2 | |
|||
| 11. Jornada | 05.12.2020 | AFC Ajax | - | FC Twente | 1:2 | ||||
| 12. Jornada | 13.12.2020 | FC Twente | - | AZ Alkmaar | 1:3 | ||||
| 13. Jornada | 19.12.2020 | VVV-Venlo | - | FC Twente | 1:2 | ||||
| 14. Jornada | 22.12.2020 | FC Twente | - | Sparta Rotterdam | 0:2 | ||||
| 15. Jornada | 09.01.2021 | FC Emmen | - | FC Twente | 1:4 | |
|||
| 16. Jornada | 14.01.2021 | FC Twente | - | AFC Ajax | 1:3 |
República Checa