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| 24.03.2010 | 17:30 | Bohemians Praha | - | Baník Ostrava | 0:3 (0:2) | |
| 17:30 | Dynamo České Budějovice | - | Bohemians 1905 | 0:0 (0:0) | ||
| 17:30 | Sigma Olomouc | - | FK Mladá Boleslav | 1:2 (1:0) | ||
| 17:30 | Viktoria Plzeň | - | 1. FK Příbram | 3:1 (0:0) | ||
| 17:30 | 1. FC Brno | - | SK Kladno | 3:0 (1:0) | ||
| 20:00 | FK Baumit Jablonec | - | Sparta Praga | 0:0 (0:0) | ||
| 25.03.2010 | 18:00 | FK Teplice | - | 1. FC Slovácko | 2:1 (0:0) | |
| 19:00 | Slavia Praga | - | Slovan Liberec | 0:2 (0:2) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praga | 21 | 11 | 10 | 0 | 27:9 | 18 | 43 |
| 2 |
|
FK Teplice | 21 | 12 | 7 | 2 | 35:20 | 15 | 43 |
| 3 |
|
FK Baumit Jablonec | 21 | 12 | 5 | 4 | 24:17 | 7 | 41 |
| 4 |
|
Baník Ostrava | 21 | 11 | 6 | 4 | 31:18 | 13 | 39 |
| 5 |
|
Viktoria Plzeň | 21 | 11 | 6 | 4 | 29:17 | 12 | 39 |
| 6 |
|
Slavia Praga | 21 | 8 | 7 | 6 | 26:21 | 5 | 31 |
| 7 |
|
1. FC Brno | 21 | 8 | 6 | 7 | 21:23 | -2 | 30 |
| 8 |
|
FK Mladá Boleslav | 21 | 8 | 5 | 8 | 33:29 | 4 | 29 |
| 9 |
|
Sigma Olomouc | 21 | 8 | 4 | 9 | 32:26 | 6 | 28 |
| 10 |
|
1. FK Příbram | 21 | 7 | 4 | 10 | 23:28 | -5 | 25 |
| 11 |
|
Slovan Liberec | 21 | 7 | 3 | 11 | 23:29 | -6 | 24 |
| 12 |
|
Bohemians 1905 | 21 | 6 | 6 | 9 | 13:19 | -6 | 24 |
| 13 |
|
Dynamo České Budějovice | 21 | 4 | 8 | 9 | 17:25 | -8 | 20 |
| 14 |
|
1. FC Slovácko | 21 | 5 | 3 | 13 | 19:30 | -11 | 18 |
| 15 |
|
SK Kladno | 21 | 4 | 2 | 15 | 14:35 | -21 | 14 |
| 16 |
|
Bohemians Praha | 21 | 4 | 2 | 15 | 20:41 | -21 | -6 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
