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| 27.03.2010 | 15:45 | FK Mladá Boleslav | - | Viktoria Plzeň | 4:0 (2:0) | |
| 28.03.2010 | 15:00 | Slovan Liberec | - | FK Baumit Jablonec | 4:2 (3:2) | |
| 17:00 | 1. FC Slovácko | - | Dynamo České Budějovice | 1:0 (0:0) | ||
| 17:00 | Bohemians 1905 | - | FK Teplice | 1:1 (0:1) | ||
| 17:00 | 1. FK Příbram | - | Bohemians Praha | 3:2 (1:0) | ||
| 17:00 | SK Kladno | - | Sigma Olomouc | 2:3 (0:0) | ||
| 17:00 | Sparta Praga | - | 1. FC Brno | 3:0 (1:0) | ||
| 29.03.2010 | 17:30 | Baník Ostrava | - | Slavia Praga | 3:1 (2:1) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praga | 22 | 12 | 10 | 0 | 30:9 | 21 | 46 |
| 2 |
|
FK Teplice | 22 | 12 | 8 | 2 | 36:21 | 15 | 44 |
| 3 |
|
Baník Ostrava | 22 | 12 | 6 | 4 | 34:19 | 15 | 42 |
| 4 |
|
FK Baumit Jablonec | 22 | 12 | 5 | 5 | 26:21 | 5 | 41 |
| 5 |
|
Viktoria Plzeň | 22 | 11 | 6 | 5 | 29:21 | 8 | 39 |
| 6 |
|
FK Mladá Boleslav | 22 | 9 | 5 | 8 | 37:29 | 8 | 32 |
| 7 |
|
Sigma Olomouc | 22 | 9 | 4 | 9 | 35:28 | 7 | 31 |
| 8 |
|
Slavia Praga | 22 | 8 | 7 | 7 | 27:24 | 3 | 31 |
| 9 |
|
1. FC Brno | 22 | 8 | 6 | 8 | 21:26 | -5 | 30 |
| 10 |
|
1. FK Příbram | 22 | 8 | 4 | 10 | 26:30 | -4 | 28 |
| 11 |
|
Slovan Liberec | 22 | 8 | 3 | 11 | 27:31 | -4 | 27 |
| 12 |
|
Bohemians 1905 | 22 | 6 | 7 | 9 | 14:20 | -6 | 25 |
| 13 |
|
1. FC Slovácko | 22 | 6 | 3 | 13 | 20:30 | -10 | 21 |
| 14 |
|
Dynamo České Budějovice | 22 | 4 | 8 | 10 | 17:26 | -9 | 20 |
| 15 |
|
SK Kladno | 22 | 4 | 2 | 16 | 16:38 | -22 | 14 |
| 16 |
|
Bohemians Praha | 22 | 4 | 2 | 16 | 22:44 | -22 | -6 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
