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| 03.04.2010 | 15:45 | FK Baumit Jablonec | - | Sigma Olomouc | 3:1 (2:1) | |
| 18:00 | 1. FC Slovácko | - | Slovan Liberec | 0:0 (0:0) | ||
| 18:00 | 1. FC Brno | - | FK Mladá Boleslav | 2:3 (1:2) | ||
| 04.04.2010 | 17:00 | Bohemians Praha | - | SK Kladno | 1:4 (0:2) | |
| 17:00 | Dynamo České Budějovice | - | 1. FK Příbram | 2:1 (0:0) | ||
| 05.04.2010 | 15:30 | Slavia Praga | - | Bohemians 1905 | 3:0 (0:0) | |
| 15:30 | FK Teplice | - | Baník Ostrava | 0:1 (0:1) | ||
| 17:30 | Viktoria Plzeň | - | Sparta Praga | 2:2 (0:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta | 1.ª parte | 2.ª parte |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praga | 23 | 12 | 11 | 0 | 32:11 | 21 | 47 |
| 2 |
|
Baník Ostrava | 23 | 13 | 6 | 4 | 35:19 | 16 | 45 |
| 3 |
|
FK Teplice | 23 | 12 | 8 | 3 | 36:22 | 14 | 44 |
| 4 |
|
FK Baumit Jablonec | 23 | 13 | 5 | 5 | 29:22 | 7 | 44 |
| 5 |
|
Viktoria Plzeň | 23 | 11 | 7 | 5 | 31:23 | 8 | 40 |
| 6 |
|
FK Mladá Boleslav | 23 | 10 | 5 | 8 | 40:31 | 9 | 35 |
| 7 |
|
Slavia Praga | 23 | 9 | 7 | 7 | 30:24 | 6 | 34 |
| 8 |
|
Sigma Olomouc | 23 | 9 | 4 | 10 | 36:31 | 5 | 31 |
| 9 |
|
1. FC Brno | 23 | 8 | 6 | 9 | 23:29 | -6 | 30 |
| 10 |
|
Slovan Liberec | 23 | 8 | 4 | 11 | 27:31 | -4 | 28 |
| 11 |
|
1. FK Příbram | 23 | 8 | 4 | 11 | 27:32 | -5 | 28 |
| 12 |
|
Bohemians 1905 | 23 | 6 | 7 | 10 | 14:23 | -9 | 25 |
| 13 |
|
Dynamo České Budějovice | 23 | 5 | 8 | 10 | 19:27 | -8 | 23 |
| 14 |
|
1. FC Slovácko | 23 | 6 | 4 | 13 | 20:30 | -10 | 22 |
| 15 |
|
SK Kladno | 23 | 5 | 2 | 16 | 20:39 | -19 | 17 |
| 16 |
|
Bohemians Praha | 23 | 4 | 2 | 17 | 23:48 | -25 | -6 |
| CL Ronda previa | |
| Europa League | |
| Descenso |
