| 1. Jornada | 22.08.2021 | Benevento Calcio | - | US Alessandria 1912 | 4:3 | ||||
| 2. Jornada | 27.08.2021 | Pisa SC | - | US Alessandria 1912 | 2:0 | ||||
| 5. Jornada | 21.09.2021 | US Alessandria 1912 | - | Ascoli Calcio | 1:3 | ||||
| 9. Jornada | 22.10.2021 | US Alessandria 1912 | - | FC Crotone | 1:0 | ||||
| 10. Jornada | 28.10.2021 | US Alessandria 1912 | - | Frosinone | 1:1 | ||||
| 11. Jornada | 01.11.2021 | AC Monza | - | US Alessandria 1912 | 1:0 | ||||
| 12. Jornada | 06.11.2021 | US Alessandria 1912 | - | Ternana Calcio | 0:2 | ||||
| 13. Jornada | 20.11.2021 | SPAL 2013 Ferrara | - | US Alessandria 1912 | 2:3 |  |
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| 14. Jornada | 27.11.2021 | US Alessandria 1912 | - | US Cremonese | 1:0 |  |
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| 15. Jornada | 30.11.2021 | Pordenone Calcio | - | US Alessandria 1912 | 2:0 | ||||
| 16. Jornada | 05.12.2021 | US Alessandria 1912 | - | AS Cittadella | 0:1 | ||||
| 17. Jornada | 12.12.2021 | Reggina 1914 | - | US Alessandria 1912 | 0:4 | ||||
| 18. Jornada | 19.12.2021 | US Alessandria 1912 | - | Parma Calcio 1913 | 0:2 |
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